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Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds.

Rui Hong·Nicholas O Fischer·Ayush Verma·Catherine M Goodman·Todd Emrick·Vincent M Rotello
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Abstract

Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.

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Cadmium CompoundsChymotrypsinCircular DichroismFatty AcidsNanotechnologyOsmolar ConcentrationPolyethylene GlycolsProtein ConformationSelenium CompoundsSurface PropertiesJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Rui Hong, Nicholas O Fischer, Ayush Verma, Catherine M Goodman, Todd Emrick, Vincent M Rotello. 2004-01-28. Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds.. https://doi.org/10.1021/ja037470o

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