PubMed · 14748796
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P Osin. 2004. Abolish C3.. https://doi.org/10.1111/j.1365-2303.2003.00116.x
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BACKGROUND: The majority of patients with lymphoma undergo a single biopsy for diagnosis, and there are few opportunities to acquire posttreatment material. Fine-needle aspiration (FNA) biopsy is a minimally invasive procedure, and acquiring routine posttreatment material would require minimal effort and provide needed material for gene expression profiling. METHODS: Ex vivo FNA biopsies were performed using a standard clinical technique with 21-gauge, 22-gauge, 23-gauge, and 25-gauge needles for 8 lymph node specimens and were collected in either RNA stabilization reagent (RNAlater) or Trizol. Eight patients with known or suspected Non-Hodgkin lymphoma (NHL) underwent interoperative (in vivo) FNA biopsies based on the best technique derived from the ex vivo aspirates. RNA derived from the in vivo FNA biopsies and the matched, snap-frozen surgical lymph node biopsy remnant was used for gene expression analysis with proprietary U133A chips. RESULTS: The results confirmed the authors' experience, that RNA isolated from FNA biopsies of lymph nodes collected in Trizol is superior both quantitatively and qualitatively to RNA collected in RNAlater. Gene expression profiles of NHL derived from in vivo FNA biopsies and matched, frozen surgical specimens showed good overall correlation. CONCLUSIONS: High-throughput gene expression analysis in patients with NHL derived from material acquired by FNA biopsy is a feasible approach to developing a platform for real-time analysis of treatment responses in this group of patients.
A 3-month-old male with a chief complaint of episodic choking with feeds and a hoarse cry is presented. Left eye ptosis and asymmetric soft palate elevation were detected on physical examination. Fiberoptic examination showed a left vocal fold paresis and pooling of secretions in the pyriform sinuses. MRI demonstrated an ill-defined lesion at the left jugular foramen extending into the left carotid sheath. A fine needle biopsy revealed spindle shaped cells consistent with fibromatosis. The histopathology of fibromatosis and the differential diagnosis of jugular foramen masses in children will be described. To our knowledge, this represents the earliest reported case of fibromatosis in the jugular foramen.
In a 55-year-old woman, an echographically occult breast tumour was only visible in one direction on mammography. MRI-guided needle localisation of the lesion followed by surgical excision biopsy were then carried out for diagnostic purposes. In a second woman, 61 years of age, mammographic needle localisation of a deep, echographically occult, lesion was unsuccessful. She then also underwent MRI-guided needle localisation followed by surgical excision biopsy. A third woman, also 61 years of age, with known carcinoma underwent an MRI staging study. This revealed a second, mammographically and echographically occult, lesion for which MRI-guided large-core needle biopsy was done. MRI of the breast has a high sensitivity for the detection of invasive breast carcinoma. MRI of the breast reveals suspicious lesions that cannot be seen on the conventional mammogram or by echography in 15-25% of the cases. In order to obtain tissue from these lesions for histopathology, MRI-guided biopsy techniques have been developed. The most commonly used methods are MRI-guided needle localisation followed by surgical excision biopsy and MRI-guided large-core needle biopsy. The demand for MRI-guided breast interventions is growing because of the increasing use of MRI of the breast in clinical practice.