A polymer scaffold for protein oligomerization.

Byron R Griffith; Benjamin L Allen; Alan C Rapraeger; Laura L Kiessling

doi:10.1021/ja037646m

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A polymer scaffold for protein oligomerization.

Byron R Griffith·Benjamin L Allen·Alan C Rapraeger·Laura L Kiessling

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Abstract

We report the design and synthesis of well-defined polymers for the noncovalent oligomerization of proteins. The reported scaffolds, which were generated by atom-transfer radical polymerization (ATRP), take advantage of the well-characterized interaction between a Ni2+ complex and an oligohistidine sequence (His tag). Thus, our polymers are designed to facilitate the oligomerization of any protein possessing a His tag. We demonstrate that they can oligomerize fibroblast growth factor-8b (FGF-8b) and promote FGF-8b-mediated cell proliferation in the absence of heparin.

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Animals Cell Division Cell Line Chelating Agents Fibroblast Growth Factor 8 Fibroblast Growth Factors Histidine Mice Nickel Polymers Proteins Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Byron R Griffith, Benjamin L Allen, Alan C Rapraeger, Laura L Kiessling. 2004-02-18. A polymer scaffold for protein oligomerization.. https://doi.org/10.1021/ja037646m

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