PubMed · 14878711
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I F RUDAKOV. [Volatile substance in Clematis].. https://pubmed.ncbi.nlm.nih.gov/14878711/
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OBJECTIVE: To study the chemical constituents from the roots and rhizomes of Clematis hexapetala. METHOD: The compounds were separated by means of solvent extraction, repeated chromatography with silica gel and HPLC. The structures were determined by spectral analysis. RESULT: Nine compounds were isolated as friedelin (1) , anemonin (2) , beta-sitosterol (3) , palmitic acid (4) , vanillic acid (5) , isolariciresinol (6) , 5-hydroxumethyl-5H-furan-2-one (7) , n-nonane (8) , daucosterol (9). CONCLUSION: All the compounds were isolated from the plant for the first time.
The rapid propagation of Clematis filamentosa Dumn by tissue cluture showed that the best explant was young stem. It also showed that the callus can be easily induced in MS + NAA 0.1 mg/L + 6-BA 0.5 mg/L, the medium for the buds differentiation and proliferation was MS + NAA 0.05 mg/L + 6-BA 0.5 mg/L, and the medium for the root growth was MS + NAA 0.1 mg/L. The survival tube seedling can be successfully transplant.
Genome variability of in vitro micropropagated Clematis plants was established. The optimum concentrations of BAP and zeatin in the culture medium regulating in vitro morphogenetic processes in clematis explants cv. Serenada Kryma were determined. Molecular-genetic analysis of Clematis plants obtained via in vitro somatic embryogenesis and organogenesis was carried out. Using ISSR primers 105 amplicons have been revealed, six of them were polymorphic. The mean index of heterogeneity of clematis plants was 5.7%.