Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability.

Dania A Fonseca; Humberto R Gutiérrez; Kenneth E Collins; Carol H Collins

doi:10.1016/j.chroma.2003.12.033

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Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability.

Dania A Fonseca·Humberto R Gutiérrez·Kenneth E Collins·Carol H Collins

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Abstract

A procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60 degrees C. Mobile phase (MeOH-0.1 mol l(-1) aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process.

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Dania A Fonseca, Humberto R Gutiérrez, Kenneth E Collins, Carol H Collins. 2004-03-19. Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability.. https://doi.org/10.1016/j.chroma.2003.12.033

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