Headspace solid-phase micro-extraction and gas chromatography with micro-electron capture detection for the measurement of p,p'-DDE in rat whole blood and hair.

Takahiko Kusakabe; Takeshi Saito; Sanae Takeichi

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PubMed · 15055406

Headspace solid-phase micro-extraction and gas chromatography with micro-electron capture detection for the measurement of p,p'-DDE in rat whole blood and hair.

Takahiko Kusakabe·Takeshi Saito·Sanae Takeichi

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Abstract

A sensitive, specific, and reproducible capillary gas chromatography (GC) with micro-electron capture detection (micro-ECD) method using headspace solid-phase micro-extraction (HS-SPME) for the quantitative analysis of p,p'-DDE in rat whole blood and hair was developed. A 100 microm polydimethylsiloxane (PDMS) phase was used for the extraction. The obtained detection limits were 0.003 ng/mg and 0.004 ng/mg in whole blood and hair, respectively, at a signal-to-noise ratio of 3 (S/N = 3). Linearity was obtained in the ranges 0.003-2.0 ng/mg and 0.004-2.0 ng/mg in whole blood and hair, respectively, and the correlation coefficients of whole blood and hair were greater than 0.998. This method was successfully applied to the analysis of p,p'-DDE in rat whole blood and rat hair after an oral dose of p,p'-DDE.

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Animals Blood Chromatography, Gas Dichlorodiphenyl Dichloroethylene Hair Insecticides Molecular Structure Rats Reproducibility of Results Sensitivity and Specificity Temperature Journal Article

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Takahiko Kusakabe, Takeshi Saito, Sanae Takeichi. 2003. Headspace solid-phase micro-extraction and gas chromatography with micro-electron capture detection for the measurement of p,p'-DDE in rat whole blood and hair.. https://pubmed.ncbi.nlm.nih.gov/15055406/

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