PubMed · 15058610
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George A Papazian. 2004. Total-etch versus self-etch.. https://doi.org/10.14219/jada.archive.2004.0164
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The structural integrity of fibrillar type I collagen is critical for effective dentin bonding. Since most noncollagenous matrix components in dentin are closely associated with collagen, we hypothesized that they may also contribute to dentin bonding. To test this hypothesis, bovine dentin was acid-etched, treated with chondroitinase ABC (C-ABC), endo-beta-galactosidase (Endo-beta), or trypsin. Controls were prepared in the same manner but without the enzymes. All control and experimental specimens were then bonded with One-Step. Bond strength data were analyzed by one-way ANOVA and Fisher's PLSD test (p < 0.05). When dentin was treated with C-ABC or trypsin, bond strengths significantly decreased for the rewetted groups (p < 0.05). The treatment with Endo-beta showed no effects on bond strengths (p > 0.05). When the treated dentin surfaces were observed under SEM, the C-ABC and trypsin treated groups revealed significant loss of collagen fibril architecture. The results indicate that chondroitin sulfate glycosaminoglycans and trypsin-digestible noncollagenous proteins play roles in maintaining the open dimensions of the collagen fibril scaffold, which is essential for optimal dentin bonding.
Although 4-META/MMA-TBB resin has adhesive properties to dentin, and has been clinically used for the bonding treatment of vertically fractured roots and apicoectomy, there has not been any investigation on the adhesion of 4-META/MMA-TBB resin to cementum. The purpose of this in vitro study was to evaluate the bonding and the sealing ability of 4-META/MMA-TBB resin to cementum. Bovine root cementum and dentin surfaces were treated with a citric acid and ferric chloride solution, and the 4-META/MMA-TBB resin was applied on the treated surfaces before testing. The microtensile bond strength and the leakage levels obtained for the cementum were almost equal to those for the dentin. In SEM and TEM observations, a hybrid layer approximately 2-3 microm in thickness was observed at the interface between the resin and the cementum. It is concluded that 4-META/MMA-TBB resin adhered to cementum via a hybrid layer on cementum, as previously reported for dentin.
OBJECTIVES: This study examined the effect of air-drying on bond strength to dentin and nanoleakage in resin-dentin interfaces using HEMA-free one-step self-etch adhesives. METHODS: Twelve teeth had their occlusal dentin exposed and split in half. In the group of G-BOND (GB), the adhesive was dried gently or vigorously on each slit half tooth, and then light-cured. In the group of the experimental adhesive (EX), three coats of adhesive were dried using a single air-drying process prior to light-curing or dried after each of three coats of adhesive before light-curing. The split specimens were built-up with resin composite and then subjected to microtensile bond strength testing and TEM examination. RESULTS: GB showed significantly higher bond strength after vigorous drying than after gentle drying. Three teeth of from EX group showed higher bond strength after triple drying than after single drying, while two teeth showed no significant difference. Droplets were revealed in the adhesive layer after gentle drying with GB, and silver deposits existed along the interfaces in all groups. SIGNIFICANCE: The bonding quality to dentin may be improved by using an accelerated air-drying technique; although it is impossible to remove intrinsic dentin moisture that is drawn upwards from dentin tubules.