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Three-dimensional cellular-level imaging using full-field optical coherence tomography.

A Dubois·G Moneron·K Grieve·A C Boccara
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Abstract

An ultrahigh-resolution full-field optical coherence tomography (OCT) system has been developed for cellular-level imaging of biological media. The system is based on a Linnik interference microscope illuminated with a tungsten halogen lamp, associated with a high-resolution CCD camera. En face tomographic images are produced in real time, with the best spatial resolution ever achieved in OCT (0.7 microm x 0.9 microm, axial x transverse). A shot-noise limited detection sensitivity of 80 dB can be reached with an acquisition time per image of 1 s. Images of animal ophthalmic biopsies and vegetal tissues are shown.

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AnimalsEyeImage EnhancementImage Interpretation, Computer-AssistedImaging, Three-DimensionalMicroscopy, InterferencePlant LeavesRatsNicotianaTomography, Optical CoherenceComparative StudyEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov'tValidation Study

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A Dubois, G Moneron, K Grieve, A C Boccara. 2004-04-07. Three-dimensional cellular-level imaging using full-field optical coherence tomography.. https://doi.org/10.1088/0031-9155%2F49%2F7%2F010

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