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Headspace solid-phase microextraction and capillary gas chromatographic-mass spectrometric determination of rivastigmine in canine plasma samples.

Yunfei Sha·Chunhui Deng·Zhen Liu·Taomin Huang·Bei Yang·Gengli Duan
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Abstract

A simple, rapid and sensitive method for determination of rivastigmine in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC-MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-microm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5 ml of plasma modified with 1.0 ml of sodium hydroxide-sodium carbonate solution (0.7 M:0.5M); extraction temperature of 100 degrees C, with stirring at 2000 rpm for 30 min. The calibration curve showed linearity in the range from 0.2 to 80 ng/ml with regression coefficient corresponding to 0.9965 and coefficient of the variation of the points of the calibration curve lower than 10%. The quantification limit for rivastigmine in plasma was 0.2 ng/ml. The method was applied to determination of rivastigmine in canine plasma samples from animals after a single oral administration.

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AnimalsCholinesterase InhibitorsDogsGas Chromatography-Mass SpectrometryNeuroprotective AgentsPhenylcarbamatesRivastigmineJournal ArticleResearch Support, Non-U.S. Gov't

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Yunfei Sha, Chunhui Deng, Zhen Liu, Taomin Huang, Bei Yang, Gengli Duan. 2004-07-05. Headspace solid-phase microextraction and capillary gas chromatographic-mass spectrometric determination of rivastigmine in canine plasma samples.. https://doi.org/10.1016/j.jchromb.2004.04.006

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