Single-base mutation detection using neutravidin-modified polystyrene nanoparticle aggregation.

Kae Sato; Munetaka Sawayanagi; Kazuo Hosokawa; Mizuo Maeda

doi:10.2116/analsci.20.893

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Single-base mutation detection using neutravidin-modified polystyrene nanoparticle aggregation.

Kae Sato·Munetaka Sawayanagi·Kazuo Hosokawa·Mizuo Maeda

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Abstract

A single-base mutation assay using the non-crosslinking aggregation of neutravidin-modified polystyrene nanoparticles is described. This method requires only two steps: hybridization of biotinylated probe and sample DNAs, and then mixing with neutravidin-modified nanoparticles. The aggregation was detected within 20 min in total. A combination of the DNA non-crosslinking aggregation and biotin-avidin technology has allowed sufficient performance for the detection of single nucleotide polymorphisms.

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Kae Sato, Munetaka Sawayanagi, Kazuo Hosokawa, Mizuo Maeda. 2004. Single-base mutation detection using neutravidin-modified polystyrene nanoparticle aggregation.. https://doi.org/10.2116/analsci.20.893

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