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PubMed · 15307448

Surface effects on PCR reactions in multichip microfluidic platforms.

Nicholas J Panaro·Xing Jian Lou·Paolo Fortina·Larry J Kricka·Peter Wilding
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Abstract

We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.

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Equipment DesignEquipment Failure AnalysisMaterials TestingMicrofluidic Analytical TechniquesPlasticsPolymerase Chain ReactionSurface PropertiesComparative StudyEvaluation StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Nicholas J Panaro, Xing Jian Lou, Paolo Fortina, Larry J Kricka, Peter Wilding. 2004. Surface effects on PCR reactions in multichip microfluidic platforms.. https://doi.org/10.1023/b%3Abmmd.0000013369.34716.8d

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