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Enantioselective biocatalysis optimized by directed evolution.

Karl-Erich Jaeger·Thorsten Eggert
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Abstract

Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactions: the development of whole-cell biocatalysts through the creation of designer organisms; the optimization of enzymes with existing enantioselectivity for process conditions; and the evolution of novel enantioselective biocatalysts starting from non-selective wild-type enzymes.

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AlgorithmsBiotechnologyCatalysisDirected Molecular EvolutionDrug DesignEnzymesEvolution, MolecularGene Expression ProfilingIsomerismModels, GeneticMutagenesis, Site-DirectedProtein EngineeringRecombinant ProteinsSequence Analysis, ProteinJournal ArticleResearch Support, Non-U.S. Gov'tReview

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BibTeXRIS

Karl-Erich Jaeger, Thorsten Eggert. 2004. Enantioselective biocatalysis optimized by directed evolution.. https://doi.org/10.1016/j.copbio.2004.06.007

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