A novel phosphoprotein is induced during bone marrow commitment to dendritic cells.

Nisreen Al-Shaibi; Swapan K Ghosh

doi:10.1016/j.bbrc.2004.06.106

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A novel phosphoprotein is induced during bone marrow commitment to dendritic cells.

Nisreen Al-Shaibi·Swapan K Ghosh

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Abstract

Dendritic cells (DCs) play an important role in vertebrate immunity, but little is known of the molecular events associated with their development from bone marrow (BM). This report describes induction of a signature protein marking BM commitment to DCs. Using a standard procedure, DCs were generated from BM by cultivation in vitro. Appropriate phenotypic monitoring was done primarily by immunofluorescence, and polyclonal antibody reagents were developed against immature DC lysates. Using one specific antibody reagent, we identified, purified, and sequenced a unique cytosolic phosphoprotein DP58 that occurs within 30 min during BM commitment to DCs. Its sequence matches with a computationally predicted Riken cDNA (GenBank Accession No. XP_138799), and a specific anti-DP58 peptide antibody was developed for further characterization. The study suggests that DP58 induction signals distinct pathway(s) leading to early DC progenitors that may be generated and propagated for a short period in vitro.

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Amino Acid Sequence Animals Antigens, Surface Bone Marrow Cells Cell Differentiation Cells, Cultured Dendritic Cells Immunophenotyping Mice Mice, Inbred BALB C Molecular Sequence Data Molecular Weight Phosphoproteins Rabbits Stem Cells Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Nisreen Al-Shaibi, Swapan K Ghosh. 2004-08-13. A novel phosphoprotein is induced during bone marrow commitment to dendritic cells.. https://doi.org/10.1016/j.bbrc.2004.06.106

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