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Novel pulse duplicating bioreactor system for tissue-engineered vascular construct.

Yuji Narita·Ken-Ichiro Hata·Hideaki Kagami·Akihiko Usui·Minoru Ueda·Yuichi Ueda
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Abstract

Cell culture in a biomimetic environment is known to improve the mechanical endurance of tissue-engineered cardiovascular components. Our goal was to generate a bioreactor that can reproduce a wide range of pulsatile flows with a completely physiological pressure profile. The morphology and biochemical properties of tissue-engineered products were also studied to test the usefulness of this novel bioreactor. The combination of an outflow valve, compliance chamber, and resistant clamps together with a balloon pumping system was able to successfully reproduce both physiological systolic and diastolic pressures. The compliance chamber was especially effective in transforming the original peaky pressure waveform into a physiological pressure profile. The tissues, cultured under a physiological pressure waveform with pulsatile flow, presented widely distributed cells in close contact with each other. They also showed significantly higher cell numbers, total protein content, and proteoglycan-glycosaminoglycan content than cultured tissues under a peaky pressure wave or under static conditions. This new bioreactor system is suitable for evaluating a favorable environment for tissue-engineered cardiovascular components.

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AnimalsBioartificial OrgansBioreactorsBlood Vessel ProsthesisCell ProliferationCell SurvivalCells, CulturedDogsEndothelial CellsEquipment DesignEquipment Failure AnalysisMechanotransduction, CellularMicrofluidicsPressurePulsatile FlowTissue Culture TechniquesTissue EngineeringEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Yuji Narita, Ken-Ichiro Hata, Hideaki Kagami, Akihiko Usui, Minoru Ueda, Yuichi Ueda. Novel pulse duplicating bioreactor system for tissue-engineered vascular construct.. https://doi.org/10.1089/ten.2004.10.1224

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