A new method for quantitative cellular imaging on 3-D scaffolds using fluorescence microscopy.

Maria Filomena Santarelli; Lorenzo Sani; Arti Ahluwalia; Giovanni Vozzi; Luigi Landini; Danilo De Rossi

doi:10.1109/tnb.2003.813938

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A new method for quantitative cellular imaging on 3-D scaffolds using fluorescence microscopy.

Maria Filomena Santarelli·Lorenzo Sani·Arti Ahluwalia·Giovanni Vozzi·Luigi Landini·Danilo De Rossi

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Abstract

This paper presents a new image processing technique for estimating cell numbers and contours in three-dimensional (3-D) microfabricated scaffolds. The method is based on a statistical approach, and utilizes the extreme value theory, which assumes that cells in the image field are rare, high-intensity events. Confocal microscopy images of fibroblasts on 3-D structures were processed using the method, and the resulting data on cell numbers was compared with countings obtained using a Burker chamber. The results were identical to within a few percent.

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3T3 Cells Algorithms Animals Cell Count Cell Culture Techniques Cell Size Image Interpretation, Computer-Assisted Imaging, Three-Dimensional Membranes, Artificial Mice Microscopy, Confocal Microscopy, Fluorescence Reproducibility of Results Sensitivity and Specificity Tissue Engineering Comparative Study Evaluation Study Journal Article Validation Study

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Maria Filomena Santarelli, Lorenzo Sani, Arti Ahluwalia, Giovanni Vozzi, Luigi Landini, Danilo De Rossi. 2003. A new method for quantitative cellular imaging on 3-D scaffolds using fluorescence microscopy.. https://doi.org/10.1109/tnb.2003.813938

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