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PubMed · 1545867

Genetic immunization is a simple method for eliciting an immune response.

D C Tang·M DeVit·S A Johnston
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Abstract

To produce an immune reaction against a foreign protein usually requires purification of that protein, which is then injected into an animal. The isolation of enough pure protein is time-consuming and sometimes difficult. Here we report that such a response can also be elicited by introducing the gene encoding a protein directly into the skin of mice. This is achieved using a hand-held form of the biolistic system which can propel DNA-coated gold microprojectiles directly into cells in the living animal. Genetic immunization may be time- and labour-saving in producing antibodies and may offer a unique method for vaccination.

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AnimalsAntibodiesAntibody FormationAntigensDNA, RecombinantFemaleGrowth HormoneHumansImmunizationLuciferasesMaleMiceMice, Inbred C57BLMice, Inbred ICRPlasmidsSkinTransfectionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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D C Tang, M DeVit, S A Johnston. 1992-03-12. Genetic immunization is a simple method for eliciting an immune response.. https://doi.org/10.1038/356152a0

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