Continuous cell partitioning using an aqueous two-phase flow system in microfluidic devices.

Masumi Yamada; Vivi Kasim; Megumi Nakashima; Jun'ichi Edahiro; Minoru Seki

doi:10.1002/bit.20276

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Continuous cell partitioning using an aqueous two-phase flow system in microfluidic devices.

Masumi Yamada·Vivi Kasim·Megumi Nakashima·Jun'ichi Edahiro·Minoru Seki

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Abstract

We present a novel microfluidic system in which an aqueous two-phase laminar flow is stably formed, and the continuous partitioning of relatively large cells can be performed, eliminating the influence of gravity. In this study, plant cell aggregates whose diameters were 37-96 microm were used as model particles. We first performed cell partitioning using a simple straight microchannel having two inlets and two outlets and examined the effects of the flow rate and the phase width on partitioning efficiency. Second, by using a microchannel with a pinched segment, the partitioning efficiency was successfully improved. This microscale aqueous two-phase flow system can further be incorporated into micro total analysis systems (microTAS) or lab-on-a-chip technology, owing to its simplicity, applicability, and biocompatibility.

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Cell Aggregation Cell Culture Techniques Cell Separation Cells, Cultured Equipment Design Equipment Failure Analysis Feasibility Studies Flow Cytometry Fragaria Microfluidic Analytical Techniques Phase Transition Plant Leaves Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Masumi Yamada, Vivi Kasim, Megumi Nakashima, Jun'ichi Edahiro, Minoru Seki. 2004-11-20. Continuous cell partitioning using an aqueous two-phase flow system in microfluidic devices.. https://doi.org/10.1002/bit.20276

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