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Murine Asb-17 expression during mouse testis development and spermatogenesis.

Kye-Seong Kim·Myung-Sun Kim·Soo-Kyung Kim·Kwang-Hyun Baek
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Abstract

In this study we isolated a murine mAsb-17 from mouse testis by RT-PCR using primers designed based on the sequences from the GenBank database. The sequence analysis showed that mAsb-17 encodes a 295 amino acid polypeptide with a molecular weight of approximately 34 kDa containing two ankyrin repeats and one SOCS box. The amino acid sequence of mASB-17 showed 87.5%, 98.3% and 92.9% identity with that of human, rat and dog, respectively. Interestingly, northern blot analysis showed that mAsb-17 was expressed only in the testis. The expression analysis by RT-PCR for mAsb-17 in mouse indicates that mAsb-17 is expressed from the fourth week after birth to adult, with the highest expression in round spermatids. Both northern blot and RT-PCR analyses suggest that mASB-17 may play essential roles in testis development and spermatogenesis.

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Adaptor Proteins, Signal TransducingAmino Acid SequenceAnimalsAnkyrin RepeatBase SequenceDNA, ComplementaryDogsGene Expression Regulation, DevelopmentalHumansMaleMiceMice, Inbred ICRMolecular Sequence DataRNA, MessengerRatsReverse Transcriptase Polymerase Chain ReactionSequence Homology, Amino AcidSignal TransductionSpecies SpecificitySpermatogenesisTestisTissue DistributionComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Kye-Seong Kim, Myung-Sun Kim, Soo-Kyung Kim, Kwang-Hyun Baek. 2004. Murine Asb-17 expression during mouse testis development and spermatogenesis.. https://doi.org/10.1017/s0967199404002722

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