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PubMed · 15468810

Strategy for generating tissue-engineered human bone construct.

K K Tan·B S Aminuddin·G H Tan·M Sabarul Afian·M H Angela Ng·O Fauziah·B H I Ruszymah
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Abstract

The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.

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Bone RegenerationBone TransplantationCell DivisionCollagenHumansMesenchymal Stem Cell TransplantationOrgan Culture TechniquesPeriosteumTissue EngineeringJournal Article

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K K Tan, B S Aminuddin, G H Tan, M Sabarul Afian, M H Angela Ng, O Fauziah, B H I Ruszymah. 2004. Strategy for generating tissue-engineered human bone construct.. https://pubmed.ncbi.nlm.nih.gov/15468810/

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