Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates.

Rui Hong; Todd Emrick; Vincent M Rotello

doi:10.1021/ja0461163

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Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates.

Rui Hong·Todd Emrick·Vincent M Rotello

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Abstract

Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues.

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Acetates Catalysis Chymotrypsin Ethylene Glycols Gold Nanostructures Oligopeptides Substrate Specificity Sulfhydryl Compounds Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Rui Hong, Todd Emrick, Vincent M Rotello. 2004-10-27. Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates.. https://doi.org/10.1021/ja0461163

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