What do microarrays really tell us about M. tuberculosis?

Sharon L Kendall; Stuart C G Rison; Farahnaz Movahedzadeh; Rosangela Frita; Neil G Stoker

doi:10.1016/j.tim.2004.10.005

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What do microarrays really tell us about M. tuberculosis?

Sharon L Kendall·Stuart C G Rison·Farahnaz Movahedzadeh·Rosangela Frita·Neil G Stoker

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Abstract

Bacterial pathogens adapt to their host environments to a large extent through switching on complex transcriptional programmes, and whole-genome microarray experiments promise to reveal this complexity. There has been a recent burst of articles reporting transcriptome analyses of Mycobacterium tuberculosis, including for the first time studies in macrophages and mice. We review gene expression reports, and compare them with each other and with microarray-based gene essentiality studies, revealing at times a startling lack of correlation. Additionally, we suggest a standardization format for the submission of processed data for publication, to facilitate cross-experiment analyses.

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Animals Gene Expression Profiling Gene Expression Regulation, Bacterial Mycobacterium tuberculosis Oligonucleotide Array Sequence Analysis RNA, Bacterial Journal Article Research Support, Non-U.S. Gov't Review

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Sharon L Kendall, Stuart C G Rison, Farahnaz Movahedzadeh, Rosangela Frita, Neil G Stoker. 2004. What do microarrays really tell us about M. tuberculosis?. https://doi.org/10.1016/j.tim.2004.10.005

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