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Matrix-implanted laser desorption/ionization mass spectrometry.

Alexey Novikov·Martine Caroff·Serge Della-Negra·Yvon Lebeyec·Michèle Pautrat·J Albert Schultz·Agnès Tempez·Hay-Yan J Wang·Shelley N Jackson·Amina S Woods
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Abstract

The implantation of low-velocity massive gold clusters is shown to be a method of choice for homogeneous incorporation of a metallic matrix into the near-surface region of a solid biopolymer for subsequent laser desorption/ionization (LDI) MS analysis. Matrix implanted (MI)LDI spectra from cluster-implanted pure test peptide or tissue exhibit molecular ion peaks similar to those observed by matrix-assisted LDI. Moreover, the ion emission is very reproducible from any spot on the surface of these test samples. MILDI promises to be a powerful technique for mass spectrometric analysis of native biological samples as demonstrated by the first results on rat brain tissues.

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AnimalsBrainCalibrationGoldInsulinPhospholipidsRatsReproducibility of ResultsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationJournal ArticleResearch Support, Non-U.S. Gov't

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Alexey Novikov, Martine Caroff, Serge Della-Negra, Yvon Lebeyec, Michèle Pautrat, J Albert Schultz, Agnès Tempez, Hay-Yan J Wang, Shelley N Jackson, Amina S Woods. 2004-12-15. Matrix-implanted laser desorption/ionization mass spectrometry.. https://doi.org/10.1021/ac049123i

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