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High-resolution blood-brain barrier permeability and blood volume imaging using quantitative synchrotron radiation computed tomography: study on an F98 rat brain glioma.

Jean-François Adam·Christian Nemoz·Alberto Bravin·Stefan Fiedler·Sam Bayat·Sylvie Monfraix·Gilles Berruyer·Anne Marie Charvet·Jean-François Le Bas·Hélène Elleaume·François Estève
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Abstract

The authors previously provided evidence of synchrotron radiation computed tomography (SRCT) efficacy for quantitative in vivo brain perfusion measurements using monochromatic X-ray beams. However, this technique was limited for small-animal studies by partial volume effects. In this paper, high-resolution absolute cerebral blood volume and blood-brain barrier permeability coefficient measurements were obtained on a rat glioma model using SRCT and a CCD camera (47x47 microm2 pixel size). This is the first report of in vivo high-resolution brain vasculature parameter assessment. The work gives interesting perspectives to quantify brain hemodynamic changes accurately in healthy and pathological small animals.

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AnimalsBlood-Brain BarrierBrainBrain NeoplasmsCell Line, TumorCerebrovascular CirculationDiagnostic ImagingGliomaImage Processing, Computer-AssistedPermeabilityRatsSynchrotronsTomography, X-Ray ComputedJournal Article

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Jean-François Adam, Christian Nemoz, Alberto Bravin, Stefan Fiedler, Sam Bayat, Sylvie Monfraix, Gilles Berruyer, Anne Marie Charvet, Jean-François Le Bas, Hélène Elleaume, François Estève. 2005. High-resolution blood-brain barrier permeability and blood volume imaging using quantitative synchrotron radiation computed tomography: study on an F98 rat brain glioma.. https://doi.org/10.1038/sj.jcbfm.9600017

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