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PubMed · 15690121

Cellular DNA content parameters as prognostic indicators in human astrocytomas.

Basil F El-Rayes·Camille S Norton·Wael Sakr·Zosia Maciorowski·Daryn Smith·Haline Pietraszkiewicz·Maria Del Mar Alonso·John F Ensley
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Abstract

OBJECTIVE: Clinical parameters such as grade, size and/or location of the tumor are good predictors of outcome in patients with astrocytoma. The objective of this study was to determine whether DNA content parameters have a prognostic significance for this group of tumors. METHODS: Following optimization and validation of methodology for evaluating cellular DNA content parameters (CDCP), tumor DNA ploidy and percent S phase fraction (SPF) were determined from 64 patients using formalin fixed, paraffin embedded specimens (mean coefficient of variation=4.94) obtained over a 10-year period. Median survival times correlated with grade (I/II=1154 vs. III/IV=483days, P=0.0317). Fifty-five percent of the specimens contained DNA aneuploid (DNA-A) components (average SPF=18.3%) and 45% were DNA diploid (DNA-D) (average SPF=9.6%). Survival did not correlate with overall differences in DNA ploidy (DNA-D=181 vs. DNA-A=206days, P=0.6314) when treated and untreated tumors were analyzed. However, a trend for prolonged median survival was observed in patients whose tumors were untreated with respect to cytotoxic therapy based on DNA ploidy status (DNA-D=275 vs. DNA-A=15days, P=0.3408). Survival for all patients did not correlate with median SPF (<13.5% av.=121 vs. >13.5% av.=154days, P=0.6534). CONCLUSION: DNA content parameters may correlate with the natural history and treatment outcome of newly diagnosed untreated patients with astrocytomas.

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AdultAgedAged, 80 and overAneuploidyAntineoplastic AgentsAstrocytomaCentral Nervous System NeoplasmsDNA, NeoplasmDiploidyFemaleFlow CytometryHumansMaleMiddle AgedPrognosisRadiotherapyS PhaseSurvival AnalysisJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, P.H.S.Validation Study

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Basil F El-Rayes, Camille S Norton, Wael Sakr, Zosia Maciorowski, Daryn Smith, Haline Pietraszkiewicz, Maria Del Mar Alonso, John F Ensley. 2005. Cellular DNA content parameters as prognostic indicators in human astrocytomas.. https://doi.org/10.1007/s11060-004-6044-x

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