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Quality control guidelines for testing gram-negative control strains with polymyxin B and colistin (polymyxin E) by standardized methods.

Ronald N Jones·Tamara R Anderegg·Jana M Swenson·Quality Control Working Group
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Abstract

An eight-laboratory study addressed the urgent need for quality control (QC) ranges for susceptibility determination when testing colistin (polymyxin E) and polymyxin B, two polycationic peptide antimicrobial agents, against multidrug-resistant gram-negative bacilli. For Escherichia coli ATCC 25922l, the QC ranges were as follows: for colistin, 0.25 to 1 microg/ml (11 to 17 mm), and for polymyxin B, 0.25 to 2 microg/ml (13 to 19 mm). For Pseudomonas aeruginosa ATCC 27853, the QC ranges were as follows: for colistin, 0.25 to 2 microg/ml (11 to 17 mm), and for polymyxin B, 0.25 to 2 mug/ml (14 to 18 mm). More than 97% of all reported QC results were within these proposed ranges.

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Anti-Bacterial AgentsColistinDrug Resistance, Multiple, BacterialEscherichia coliHumansLaboratoriesMicrobial Sensitivity TestsPolymyxin BPseudomonas aeruginosaQuality ControlReference StandardsJournal ArticleResearch Support, Non-U.S. Gov't

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Ronald N Jones, Tamara R Anderegg, Jana M Swenson, Quality Control Working Group. 2005. Quality control guidelines for testing gram-negative control strains with polymyxin B and colistin (polymyxin E) by standardized methods.. https://doi.org/10.1128/jcm.43.2.925-927.2005

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