Hematologic and lymphocyte immunophenotypic reference values for normal rhesus monkey (Macaca mulatta) umbilical cord blood; gravidity may play a role in study design.

Linda B Rogers; M Bernice Kaack; Michael C Henson; Terri Rasmussen; Elizabeth Henson; Ronald S Veazey; Donald J Krogstad; Billie B Davison

doi:10.1111/j.1600-0684.2005.00104.x

PubMed · 15860123

Hematologic and lymphocyte immunophenotypic reference values for normal rhesus monkey (Macaca mulatta) umbilical cord blood; gravidity may play a role in study design.

Linda B Rogers·M Bernice Kaack·Michael C Henson·Terri Rasmussen·Elizabeth Henson·Ronald S Veazey·Donald J Krogstad·Billie B Davison

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Abstract

Hematology and flow cytometry reference values for rhesus umbilical cord blood (UCB) were established in 17 healthy infant rhesus monkeys delivered by elective cesarean section 10 days preterm. The infants were born to age matched, singly caged primigravid or secundigravid dams. The hematology and flow cytometry values were determined by automated cell counter and by FACS. No significant differences were observed with respect to infant gender. With respect to gravida, the primigravid infants had a significantly higher percentage (P= 0.05) of CD20(+) B lymphocytes in UCB. These results provide useful reference values for future studies of maternal - fetal disease transmission, vaccine and drug evaluation in non-human primate pregnancy, as well as fetal programming and immune modulation, gene therapy and the use of UCB as a source of stem cells for research and transplantation. Importantly, our results suggest that maternal gravidity may be an important variable to consider.

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Animals Blood Cell Count Fetal Blood Flow Cytometry Gravidity Immunophenotyping Macaca mulatta Reference Values Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

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Linda B Rogers, M Bernice Kaack, Michael C Henson, Terri Rasmussen, Elizabeth Henson, Ronald S Veazey, Donald J Krogstad, Billie B Davison. 2005. Hematologic and lymphocyte immunophenotypic reference values for normal rhesus monkey (Macaca mulatta) umbilical cord blood; gravidity may play a role in study design.. https://doi.org/10.1111/j.1600-0684.2005.00104.x

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