Visualizing and manipulating individual protein molecules.

Miklós S Z Kellermayer

doi:10.1088/0967-3334/26/4/r02

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Visualizing and manipulating individual protein molecules.

Miklós S Z Kellermayer

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Abstract

Single-molecule imaging and manipulation techniques have evolved in the past decade from mere jaw-dropping attractions to essential laboratory tools. By applying single-molecule methods important insights otherwise unavailable have been obtained on various biomolecular systems. Constantly improving single-molecule imaging techniques keep expanding the scale of the explorable spatial detail, thereby providing possible solutions to getting around the debilitating diffraction limit present in physiological-condition structural investigations. In some areas, such as motor protein studies, single-molecule methods have become part of the routine and essential research toolkit. Entire research fields, such as single-molecule force spectroscopy, have been born. In the present review single-molecule visualization and manipulation methods are reviewed with a focus on proteins. Relevant signals and prominent applications are discussed along with experimental examples and recent important results. Finally, the perspectives of the single-molecule field are explored.

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Elasticity Image Interpretation, Computer-Assisted Micromanipulation Microscopy, Atomic Force Microscopy, Fluorescence Molecular Biology Protein Conformation Proteins Stress, Mechanical Journal Article Research Support, Non-U.S. Gov't Review

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Miklós S Z Kellermayer. 2005-04-11. Visualizing and manipulating individual protein molecules.. https://doi.org/10.1088/0967-3334%2F26%2F4%2Fr02

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