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PubMed · 15952750

Replaceable cross-linked polyacrylamide for high performance separation of proteins.

Joann J Lu·Shaorong Liu·Qiaosheng Pu
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Abstract

An alternative sieving matrix, replaceable cross-linked polyacrylamide (rCPA), was developed for sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) separation of proteins. This rCPA could be conveniently pressurized into separation capillaries under a pressure of 80 psi. SDS-CGE separations using this matrix generated high resolutions for a wide range (approximately 4 kD to approximately 300 kD) of proteins. When compared to the most frequently used sieving matrixes, the rCPA permitted the highest resolutions with comparable or increased separation speed for protein separations.

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Acrylic ResinsElectrophoresis, CapillaryElectrophoresis, Polyacrylamide GelGelsProteinsTime FactorsPolyacrylamidesJournal Article

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Joann J Lu, Shaorong Liu, Qiaosheng Pu. Replaceable cross-linked polyacrylamide for high performance separation of proteins.. https://doi.org/10.1021/pr0500206

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