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PubMed · 15971582

[Study of recombinant stem cell factor].

Jun Wu·Xin Gong·Shao-Hong Chang·Zhi-Hu Zhao·Cong-Lin Zuo·Qing-Jun Ma
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Abstract

Stem cell factor is an important hematopoietic growth factor. In this study, the human stem cell factor was produced by recombinant E. coli, and the structure and biological activity of the recombinant stem cell factor(rhSCF) was studied. It was indicated that the rhSCF was a uncovalent dimer in phosphate buffer,and had the correct mass spectra, mass peptides spectra, composition of amino acid, N-terminal sequernce, C-terminal sequence and intrachain disulfide linkages, rhSCF alone or synergy with rhG-CSF could mobilze hematopoietic progenitors to blood in monkey.

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AnimalsCell DifferentiationCells, CulturedChromatography, High Pressure LiquidHaplorhiniHematopoietic Stem CellsHumansRecombinant ProteinsSequence Analysis, ProteinSpectrometry, Mass, Fast Atom BombardmentSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStem Cell FactorEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Jun Wu, Xin Gong, Shao-Hong Chang, Zhi-Hu Zhao, Cong-Lin Zuo, Qing-Jun Ma. 2003. [Study of recombinant stem cell factor].. https://pubmed.ncbi.nlm.nih.gov/15971582/

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