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PubMed · 16000405

Restriction mapping in nanofluidic devices.

Robert Riehn·Manchun Lu·Yan-Mei Wang·Shuang Fang Lim·Edward C Cox·Robert H Austin
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Abstract

We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of approximately 1.5 kbp in 1 min using single DNA molecules.

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DNADNA Restriction EnzymesMagnesiumMicroscopyNanostructuresNanotechnologyRestriction MappingJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Robert Riehn, Manchun Lu, Yan-Mei Wang, Shuang Fang Lim, Edward C Cox, Robert H Austin. 2005-07-06. Restriction mapping in nanofluidic devices.. https://doi.org/10.1073/pnas.0503809102

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