Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner.

Jong Pil Park; Kyung-Bok Lee; Seok Jae Lee; Tae Jung Park; Min Gon Kim; Bong Hyun Chung; Zee-Won Lee; Insung S Choi; Sang Yup Lee

doi:10.1002/bit.20581

PubMed · 16028291

Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner.

Jong Pil Park·Kyung-Bok Lee·Seok Jae Lee·Tae Jung Park·Min Gon Kim·Bong Hyun Chung·Zee-Won Lee·Insung S Choi·Sang Yup Lee

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Abstract

A novel strategy for micropatterning proteins on the surface of polyhydroxyalkanoate (PHA) biopolymer by microcontact printing (microCP) is described. The substrate binding domain (SBD) of the Pseudomonas stutzeri PHA depolymerase was used as a fusion partner for specifically immobilizing proteins on PHA substrate. Enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) fused to the SBD could be specifically immobilized on the micropatterns of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Laser scanning confocal microscopic studies suggested that two fusion proteins were micropatterned in their functionally active forms. Also, antibody binding assay by surface plasmon resonance suggested that protein-protein interaction studies could be carried out using this system.

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Adsorption Binding Sites Carboxylic Ester Hydrolases Coated Materials, Biocompatible Enzymes, Immobilized Materials Testing Polymers Protein Binding Pseudomonas stutzeri Recombinant Fusion Proteins Surface Properties Journal Article Research Support, Non-U.S. Gov't

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Jong Pil Park, Kyung-Bok Lee, Seok Jae Lee, Tae Jung Park, Min Gon Kim, Bong Hyun Chung, Zee-Won Lee, Insung S Choi, Sang Yup Lee. 2005-10-20. Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner.. https://doi.org/10.1002/bit.20581

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