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Screening method for beta-lactamase substrate profiles.

H Schumacher·M Nir·B Mansa·V F Thomsen
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Abstract

A disc diffusion method, based on the idea of Klundert, for screening of substrate profiles of beta-lactamases was developed in order to perform epidemiological studies. The method was tested against 30 different reference beta-lactamases and 59 clinical isolates known to produce TEM-1, SHV-1 and BRO-1. The reproducibility and discriminating power of the disc diffusion method made it possible to differentiate between eight types of substrate profiles for the 30 reference beta-lactamases and to differentiate between TEM-1, SHV-1 and BRO-1 from clinical isolates. In combination with analytical isoelectric focusing the disc diffusion method gives a reliable identification of beta-lactamases.

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Anti-Bacterial AgentsBacteriaEnterobacterEnterobacteriaceae InfectionsEscherichia coliEscherichia coli InfectionsHumansIsoelectric FocusingKlebsiellaKlebsiella InfectionsMethodsPseudomonasReproducibility of ResultsSubstrate Specificitybeta-Lactamasesbeta-LactamsJournal Article

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H Schumacher, M Nir, B Mansa, V F Thomsen. 1992. Screening method for beta-lactamase substrate profiles.. https://doi.org/10.1111/j.1699-0463.1992.tb00900.x

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