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Liquid chromatography-diode array detection-electrospray ionisation mass spectrometry/nuclear magnetic resonance analyses of the anti-hyperglycemic flavonoid extract of Genista tenera. Structure elucidation of a flavonoid-C-glycoside.

Amélia P Rauter·Alice Martins·Carlos Borges·Joana Ferreira·Justino Jorge·Maria-Rosário Bronze·Ana V Coelho·Young H Choi·Robert Verpoorte
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Abstract

The anti-hyperglycemic flavonoid extract obtained from Genista tenera was first studied by liquid chromatography (LC)-diode array detection (DAD) which showed the presence of two major compounds. One of them was identified as genistein-7-O-glucoside. Luteolin-7-O-glucoside was detected as a minor constituent, while luteolin-7,3'-di-O-glucoside and rutin were found in trace amounts. LC-DAD-ESI-MS and NMR were used to confirm the structure of these compounds and allowed the elucidation of the structure of the unknown major compound, which is the flavonoid 5,7,4'-trihydroxyisoflavone-8-C-glucoside.

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Carbohydrate ConformationFlavonoidsGenistaGlycosidesMagnetic Resonance SpectroscopyPlant ExtractsSpectrometry, Mass, Electrospray IonizationJournal Article

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Amélia P Rauter, Alice Martins, Carlos Borges, Joana Ferreira, Justino Jorge, Maria-Rosário Bronze, Ana V Coelho, Young H Choi, Robert Verpoorte. 2005-09-30. Liquid chromatography-diode array detection-electrospray ionisation mass spectrometry/nuclear magnetic resonance analyses of the anti-hyperglycemic flavonoid extract of Genista tenera. Structure elucidation of a flavonoid-C-glycoside.. https://doi.org/10.1016/j.chroma.2005.06.046

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