Tissue engineered fetal skin constructs for paediatric burns.

Judith Hohlfeld; Anthony de Buys Roessingh; Nathalie Hirt-Burri; Pascal Chaubert; Stefan Gerber; Corinne Scaletta; Patrick Hohlfeld; Lee Ann Applegate

doi:10.1016/s0140-6736(05)67107-3

PubMed · 16139659

Tissue engineered fetal skin constructs for paediatric burns.

Judith Hohlfeld·Anthony de Buys Roessingh·Nathalie Hirt-Burri·Pascal Chaubert·Stefan Gerber·Corinne Scaletta·Patrick Hohlfeld·Lee Ann Applegate

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Abstract

Autologous skin-grafting is the gold standard for treatment of deep second and third degree burns. Available bioengineered skin products also necessitate this two-step surgical procedure. Therefore, we developed fetal skin constructs to improve healing of such degree burns. A bank of fetal skin cells was developed from one organ donation (4 cm2 of skin allowing the preparation of several million three-dimensional skin constructs, 9x12 cm, on native horse collagen). Successive fetal constructs were applied to eight patients at every change of dressing during 1-3 weeks in an outpatient setting. Complete closure was rapid (mean 15.3 days [SD 5.5]) with little hypertrophy of new skin and no retraction seen. This simple technique provided complete treatment without auto-grafting, showing that fetal skin cells might have great potential to treat burns and eventually acute and chronic wounds of other types.

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Burns Cells, Cultured Child Child, Preschool Female Fetus Humans Infant Male Skin Transplantation Tissue Banks Tissue Engineering Wound Healing Journal Article Research Support, Non-U.S. Gov't

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Judith Hohlfeld, Anthony de Buys Roessingh, Nathalie Hirt-Burri, Pascal Chaubert, Stefan Gerber, Corinne Scaletta, Patrick Hohlfeld, Lee Ann Applegate. Tissue engineered fetal skin constructs for paediatric burns.. https://doi.org/10.1016/s0140-6736(05)67107-3

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