Loading and transfer control for Northern hybridization.

R Correa-Rotter; C N Mariash; M E Rosenberg

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PubMed · 1616700

Loading and transfer control for Northern hybridization.

R Correa-Rotter·C N Mariash·M E Rosenberg

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Abstract

We report a simple and inexpensive method to quantitate loading and transfer of RNA and to examine RNA integrity for use with Northern hybridization. This technique involves quantitation by computer-assisted video densitometry of a negative photograph of 28S and 18S rRNA from ethidium bromide-stained RNA fixed to nylon membranes. This method eliminates the issue of variability of expression of "housekeeping" genes and the need for a second round of hybridization to quantitate control probes.

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Animals Blotting, Northern Data Display Densitometry Image Interpretation, Computer-Assisted Kidney Liver Microcomputers RNA, Ribosomal, 18S RNA, Ribosomal, 28S Rats Software Video Recording Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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R Correa-Rotter, C N Mariash, M E Rosenberg. 1992. Loading and transfer control for Northern hybridization.. https://pubmed.ncbi.nlm.nih.gov/1616700/

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