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Techniques necessary for multiple tracer quantitative small-animal imaging studies.

Terry L Sharp·Carmen S Dence·John A Engelbach·Pilar Herrero·Robert J Gropler·Michael J Welch
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Abstract

INTRODUCTION: An increasing number and variety of studies on rodent models are being conducted using small-animal positron emission tomography scanners. We aimed to determine if animal handling techniques could be developed to perform routine animal imaging in a timely and efficient manner and with minimal effect on animal physiology. These techniques need to be reproducible in the same animal while maintaining hemodynamic and physiological stability. METHODS: The necessary techniques include (a) the use of inhalant anesthesia, (b) arterial and venous cannulation for multiple tracer administrations and blood sampling, (c) development of small-volume analytic columns and techniques and (d) measurement of the physiological environment during the imaging session. RESULTS: We provide an example of a cardiac imaging study using four radiotracers (15O-water, 1-[11C]-acetate, 1-[11C]-palmitate and 1-[11C]-glucose) injected into normal rats. Plasma substrates, CO2 production and total metabolites were measured. The animals remained anesthetized over the entire imaging session, and their physiological state was maintained. CONCLUSION: The intrastudy stability of the physiological measurements and substrate levels and interstudy reproducibility of the measurements are reported.

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AnimalsFemaleHeartImage EnhancementPositron-Emission TomographyRadiopharmaceuticalsRatsRats, Sprague-DawleyWhole Body ImagingJournal ArticleResearch Support, N.I.H., Extramural

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Terry L Sharp, Carmen S Dence, John A Engelbach, Pilar Herrero, Robert J Gropler, Michael J Welch. 2005. Techniques necessary for multiple tracer quantitative small-animal imaging studies.. https://doi.org/10.1016/j.nucmedbio.2005.05.010

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