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Competitive binding assay for glucose based on glycodendrimer-fluorophore conjugates.

Bennett L Ibey·Hope T Beier·Rebecca M Rounds·Gerard L Coté·Vamsi K Yadavalli·Michael V Pishko
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Abstract

A new fluorescent glucose assay has been created using Alexa Fluor 647-labeled concanavalin A (Con A) and a fourth-generation PAMAM Alexa Fluor 594-labeled glycodendrimer. This assay has been shown to have a large response to glucose within the biological range and to be capable of functioning within a polymer hydrogel. In this paper, the glucose response is shown to be a single fluorophore-based quenching reaction. Data showing that the sensor is fully reversible and specific through competitive binding between the dendrimer and glucose with Con A are presented. Overall, the assay is shown to have potential over the traditional dextran-based assay because it has a larger dynamic response to physiological glucose concentrations, incorporates longer wavelength dyes that improve signal penetration through dermal tissue, and provides an internal reference in the form of a nonreactive fluorescent label.

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Binding, CompetitiveConcanavalin ACyclic AMPGlucoseOrganic ChemicalsSensitivity and SpecificityJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Bennett L Ibey, Hope T Beier, Rebecca M Rounds, Gerard L Coté, Vamsi K Yadavalli, Michael V Pishko. 2005-11-01. Competitive binding assay for glucose based on glycodendrimer-fluorophore conjugates.. https://doi.org/10.1021/ac0507901

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