Functional assays for screening GPCR targets.

William Thomsen; John Frazer; David Unett

doi:10.1016/j.copbio.2005.10.008

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Functional assays for screening GPCR targets.

William Thomsen·John Frazer·David Unett

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Abstract

G-protein-coupled receptors (GPCRs) are valuable molecular targets for drug discovery. An important aspect of the early drug discovery process is the design and implementation of high-throughput GPCR functional assays that allow the cost-effective screening of large compound libraries to identify novel drug candidates. Several functional assay kits based on fluorescence and/or chemiluminescence detection are commercially available for convenient screen development, each having advantages and disadvantages. In addition, new GPCR biosensors and high-content imaging technologies have recently been developed that hold promise for the development of functional GPCR screens in living cells.

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Animals Biological Assay Biosensing Techniques Drug Design Humans Luminescent Measurements Receptors, G-Protein-Coupled Signal Transduction Journal Article Review

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William Thomsen, John Frazer, David Unett. 2005-10-28. Functional assays for screening GPCR targets.. https://doi.org/10.1016/j.copbio.2005.10.008

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