Nucleic acid recovery from complex environmental samples.

Kevin J Purdy

doi:10.1016/s0076-6879(05)97016-x

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Nucleic acid recovery from complex environmental samples.

Kevin J Purdy

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Abstract

Effective extraction of nucleic acid from environmental samples is an essential starting point in the molecular analysis of microbial communities in the environment. However, there are many different extraction methods in the literature and deciding which one is best suited to a particular sample is very difficult. This article details the important steps and choices in deciding how to extract nucleic acids from environmental samples and gives specific details of one method that has proven very successful at extracting DNA and RNA from a range of different samples.

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Bacteriolysis Biofilms DNA, Bacterial Endopeptidase K Environmental Microbiology RNA, Bacterial Soil Microbiology Water Microbiology Journal Article

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Kevin J Purdy. 2005. Nucleic acid recovery from complex environmental samples.. https://doi.org/10.1016/s0076-6879(05)97016-x

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