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PubMed · 16262387

Three-layer composite magnetic nanoparticle probes for DNA.

Savka I Stoeva·Fengwei Huo·Jae-Seung Lee·Chad A Mirkin
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Abstract

A method for synthesizing composite nanoparticles with a gold shell, an Fe3O4 inner shell, and a silica core has been developed. The approach utilizes positively charged amino-modified SiO2 particles as templates for the assembly of negatively charged 15 nm superparamagnetic water-soluble Fe3O4 nanoparticles. The SiO2-Fe3O4 particles electrostatically attract 1-3 nm Au nanoparticle seeds that act in a subsequent step as nucleation sites for the formation of a continuous gold shell around the SiO2-Fe3O4 particles upon HAuCl4 reduction. The three-layer magnetic nanoparticles, when functionalized with oligonucleotides, exhibit the surface chemistry, optical properties, and cooperative DNA binding properties of gold nanoparticle probes, but the magnetic properties of the Fe3O4 inner shell.

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DNADNA ProbesFerrous CompoundsGoldHot TemperatureMagneticsNanostructuresNucleic Acid DenaturationOligonucleotidesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Savka I Stoeva, Fengwei Huo, Jae-Seung Lee, Chad A Mirkin. 2005-11-09. Three-layer composite magnetic nanoparticle probes for DNA.. https://doi.org/10.1021/ja055056d

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