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PubMed · 16278920

Chiral separations on multichannel microfluidic chips.

Yan Gao·Zheng Shen·Hui Wang·Zhongpeng Dai·Bingcheng Lin
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Abstract

Chiral separations of FITC-labeled basic drugs on multichannel microfluidic chips with LIF detector were investigated. A preliminary screening procedure for seven neutral CDs was performed under optimized conditions for chiral separations of three FITC-labeled drugs (baclofen, norfenefrine, and tocainide) on a mono-channel microfluidic chip. According to the results of screening, FITC-baclofen and FITC-norfenefrine as well as two chiral selectors including gamma-CD and dimethyl-beta-CD (DM-beta-CD) were selected as models to perform chiral separations on a two-channel chip. FITC-baclofen enantiomers were separated completely by gamma-CD in one channel, while resolution of FITC-norfenefrine enantiomers was achieved by DM-beta-CD in the other channel in the same run. Furthermore, the feasibility of using one chiral selector to separate multiple chiral samples was studied on a four-channel chip. These results show that multichannel chip has a potential for chiral high-throughput screening.

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BaclofenElectrophoresis, MicrochipFluorescein-5-isothiocyanateFluorescent DyesOctopamineStereoisomerismTocainidebeta-Cyclodextrinsgamma-CyclodextrinsJournal ArticleResearch Support, Non-U.S. Gov't

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Yan Gao, Zheng Shen, Hui Wang, Zhongpeng Dai, Bingcheng Lin. 2005. Chiral separations on multichannel microfluidic chips.. https://doi.org/10.1002/elps.200500283

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