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PubMed · 16285675

Probing protein tertiary structure with amidination.

Dariusz J Janecki·Richard L Beardsley·James P Reilly
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Abstract

A chemical derivatization method, amidination, that has recently been effectively employed in peptide mass spectrometry experiments is used to covalently modify lysines in several standard proteins. Protein and peptide mass spectra identify sites at which the reaction does or does not occur. This is therefore a rapid approach to elucidate solvent-accessible regions of folded proteins.

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AminationAminesAmino Acid SequenceAnimalsCarbonic Anhydrase IICattleCrystallography, X-RayCysteineHemoglobinsHumansMagnetic Resonance SpectroscopyModels, MolecularMolecular Sequence DataProtein Structure, TertiaryProteinsSolventsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationUbiquitinJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Dariusz J Janecki, Richard L Beardsley, James P Reilly. 2005-11-15. Probing protein tertiary structure with amidination.. https://doi.org/10.1021/ac050891z

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