A guide to choosing fluorescent proteins.

Nathan C Shaner; Paul A Steinbach; Roger Y Tsien

doi:10.1038/nmeth819

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A guide to choosing fluorescent proteins.

Nathan C Shaner·Paul A Steinbach·Roger Y Tsien

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Abstract

The recent explosion in the diversity of available fluorescent proteins (FPs) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPs are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPs provides a useful guide in narrowing down the options.

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Animals Fluorescent Dyes Guidelines as Topic Humans Luminescent Proteins Microscopy, Fluorescence Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

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Nathan C Shaner, Paul A Steinbach, Roger Y Tsien. 2005. A guide to choosing fluorescent proteins.. https://doi.org/10.1038/nmeth819

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