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Disposable amperometric sensor for neurotransmitters based on screen-printed electrodes modified with a thin iridium oxide film.

Abdollah Salimi·Vali Alizadeh·Richard G Compton
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Abstract

Potential cycling in the range from -0.2 to +1.2 V is used for the electrodeposition of hydrous iridium oxide films onto a screen-printed electrode from a saturated solution of alkaline iridium(III) solution. The iridium oxide redox couple shows a stable and obvious reversible redox, with the formal potential being pH dependent in the range 1-14. The properties, stability and electrochemical properties of iridium oxide films were investigated by cyclic voltammetry. A modified electrode showed excellent catalytic activity toward the oxidation of neurotransmitters (catecholamines) over a wide pH range (2-8). The electrocatalytic behavior is further exploited as a sensitive detection scheme for adrenaline and dopamine by hydrodynamic amperometry. Under the optimized conditions, the calibration curves are linear in the concentration range 0.1-70 and 0.1-15 microM for dopamine and adrenaline determination, respectively. The detection limit and sensitivity are 30 nM and 30 nA/microM for adrenaline and 15 nM and 80 nA/microM for dopamine. Finally, the analytical performance of the modified electrode was demonstrated for the elimination of interference by uric acid in catecholamines determination when present in a 1000-fold concentration excess.

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Biosensing TechniquesCatalysisDisposable EquipmentDopamineElectrochemistryElectrodesEpinephrineIridiumNeurotransmitter AgentsOxidation-ReductionUric AcidJournal Article

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Abdollah Salimi, Vali Alizadeh, Richard G Compton. 2005. Disposable amperometric sensor for neurotransmitters based on screen-printed electrodes modified with a thin iridium oxide film.. https://doi.org/10.2116/analsci.21.1275

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