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PubMed · 16334565

[Experimental study of cartilage reconstruction using directed inducing MSCs-alginate complex].

Guang-Hui Wang·Li-Hong Wang·Chuan-Yu Liang·Wen Li
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Abstract

OBJECTIVE: To evaluate the practicability of cartilage reconstruction using directed inducing bone marrow stem cells(MSCs)- alginate complex. METHODS: The MSCs were seperated by gradient centrifugation on Percoll, then were induced into chon-drogenic differentiation. After 14 days,immunohistochemical techique was applied to detect the expression of collagen type II. Then, the MSCs and calcium alginate were mixed to form a complex and were planted under the skin of nude mice. Histochemical or immuno-histochemical tests were made at 4 weeks and 8 weeks later. RESULTS: The MSCs showed the positive result of collagen type II. There were chondrcytes and cartilage lacuna at 4 weeks later, and chondrocytes and the collagen secreted by them became more at 8 weeks later. But, the MSCs without directed inducing did not show the character of the chondrocyte. CONCLUSION: The way of using directed inducing MSCs-alginate complex can form cartilage; it is possible to repair the defect of cartilage in clinical practice.

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AlginatesAnimalsBiocompatible MaterialsBone Marrow CellsCells, CulturedChondrocytesChondrogenesisCollagen Type IIGlucuronic AcidHexuronic AcidsMiceMice, NudeRandom AllocationRatsRats, Sprague-DawleyStem CellsTissue EngineeringJournal ArticleResearch Support, Non-U.S. Gov't

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Guang-Hui Wang, Li-Hong Wang, Chuan-Yu Liang, Wen Li. 2005. [Experimental study of cartilage reconstruction using directed inducing MSCs-alginate complex].. https://pubmed.ncbi.nlm.nih.gov/16334565/

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