Simultaneous observation of enzyme surface diffusion and surface reaction using microfluidic patterning of substrate surfaces.

Shaunak Roy; Jerry M Thomas; Elizabeth A Holmes; James T Kellis; A J Poulose; Channing R Robertson; Alice P Gast

doi:10.1021/ac050544p

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Simultaneous observation of enzyme surface diffusion and surface reaction using microfluidic patterning of substrate surfaces.

Shaunak Roy·Jerry M Thomas·Elizabeth A Holmes·James T Kellis·A J Poulose·Channing R Robertson·Alice P Gast

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Abstract

We present a study of the simultaneous observation of protease reaction and surface diffusion as the enzyme interacts with a model substrate surface. We use micro-fluidic patterning to decorate a bovine serum albumin substrate surface with stripes of adsorbed enzyme in the absence of physical barriers. Spreading of the enzyme from the initial striped region indicates surface diffusion, while removal of the substrate provides a measure of reactivity. Microfluidic patterning provides a means to determine the relative importance of enzyme adsorption, surface diffusion, and reaction on the rate of substrate removal.

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Animals Cattle Diffusion Enzymes Fluorescence Recovery After Photobleaching Microfluidic Analytical Techniques Serum Albumin, Bovine Subtilisin Surface Properties Journal Article Research Support, Non-U.S. Gov't

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Shaunak Roy, Jerry M Thomas, Elizabeth A Holmes, James T Kellis, A J Poulose, Channing R Robertson, Alice P Gast. 2005-12-15. Simultaneous observation of enzyme surface diffusion and surface reaction using microfluidic patterning of substrate surfaces.. https://doi.org/10.1021/ac050544p

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