Generic bioaffinity silicone surfaces.

Hong Chen; Michael A Brook; Heather D Sheardown; Yang Chen; Bettina Klenkler

doi:10.1021/bc050174b

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Generic bioaffinity silicone surfaces.

Hong Chen·Michael A Brook·Heather D Sheardown·Yang Chen·Bettina Klenkler

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Abstract

Synthetic polymer surfaces require surface modification to improve biocompatibility. A generic route to biocompatible silicone elastomers is described involving high yield surface functionalization of standard silicones with hydrosilanes, hydrosilylation using asymmetric, allyl-, NSC-terminated PEO of narrow molecular weight, and covalent modification in one step with amine-containing biological molecules including oligopeptides (YIGSR, RGDS), proteins (EGF, albumin, fibrinogen, mucin), and glycosaminoglycans (heparin). Efficient, high-density binding (e.g., 0.2 EGF molecules/nm2) was demonstrated using radiolabeling studies. The resulting surfaces were demonstrated to be biocompatible by further reaction with biomolecules, for example, thrombosis suppression on surfaces modified by heparin + ATIII, and the formation of confluent corneal epithelial cell layers on EGF, RGDS, or YIGSR surfaces.

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Biocompatible Materials Carbonates Cell Adhesion Cells, Cultured Cornea Epithelial Cells Heparin Humans Oligopeptides Polyethylene Glycols Proteins Silicone Elastomers Succinimides Journal Article Research Support, Non-U.S. Gov't

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Hong Chen, Michael A Brook, Heather D Sheardown, Yang Chen, Bettina Klenkler. Generic bioaffinity silicone surfaces.. https://doi.org/10.1021/bc050174b

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