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Comprehensive protein profiling by multiplexed capillary zone electrophoresis using cross-linked polyacrylamide coated capillaries.

Shaorong Liu·Lin Gao·Qiaosheng Pu·Joann J Lu·Xingjia Wang
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Abstract

We have recently developed a new process to create cross-linked polyacrylamide (CPA) coatings on capillary walls to suppress protein-wall interactions. Here, we demonstrate CPA-coated capillaries for high-efficiency (>2 x 10(6) plates per meter) protein separations by capillary zone electrophoresis (CZE). Because CPA virtually eliminates electroosmotic flow, positive and negative proteins cannot be analyzed in a single run. A "one-sample-two-separation" approach is developed to achieve a comprehensive protein analysis. High throughput is achieved through a multiplexed CZE system.

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Acrylic ResinsCross-Linking ReagentsElectrophoresis, CapillaryProtein BindingProteinsSpectrophotometry, UltravioletPolyacrylamidesJournal Article

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Shaorong Liu, Lin Gao, Qiaosheng Pu, Joann J Lu, Xingjia Wang. 2006. Comprehensive protein profiling by multiplexed capillary zone electrophoresis using cross-linked polyacrylamide coated capillaries.. https://doi.org/10.1021/pr050335l

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