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Echinococcus multilocularis: developmental stage-specific expression of Antigen B 8-kDa-subunits.

Wulamu Mamuti·Yasuhito Sako·Ning Xiao·Kazuhiro Nakaya·Minoru Nakao·Hiroshi Yamasaki·Marshall W Lightowlers·Philip S Craig·Akira Ito
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Abstract

Antigen B (AgB) initially found in hydatid cyst fluid of Echinococcus granulosus is a polymeric lipoprotein of 160 kDa, and is an aggregate of several different but homologous small proteins with approximately 8 kDa. Four genes encoding these 8-kDa-subunits have been identified from E. granulosus. In this study we isolated five genes encoding 8-kDa-subunits of AgB from Echinococcus multilocularis. Sequence comparison of isolated cDNA clones demonstrated that one of these five clones was completely identical to EmAgB8/1 which had been isolated previously by our group, and three of them were 94.5, 90.8, and 91.9% homologous to E. granulosus antigen B 8-kDa subunit genes, EgAgB8/2, EgAgB8/3, and EgAgB8/4, respectively. The remaining clone shared 51-58% homology with the nucleotide sequences of AgB genes. Gene-specific RT-PCR and Western blot analyses revealed that these genes were expressed in a developmentally regulated manner in E. multilocularis vesicles, protoscoleces, and immature adult worms. Possible functions of different expression manners are also discussed.

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Amino Acid SequenceAnimalsArvicolinaeBase SequenceBlotting, WesternCricetinaeCricetulusDNA PrimersDNA, ComplementaryDNA, HelminthDogsEchinococcus multilocularisGene Expression Regulation, DevelopmentalHelminth ProteinsLipoproteinsMiceMolecular Sequence DataProtein BiosynthesisRNA, HelminthReverse Transcriptase Polymerase Chain ReactionSequence AlignmentSequence Analysis, DNATranscription, GeneticJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

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Wulamu Mamuti, Yasuhito Sako, Ning Xiao, Kazuhiro Nakaya, Minoru Nakao, Hiroshi Yamasaki, Marshall W Lightowlers, Philip S Craig, Akira Ito. 2006-02-03. Echinococcus multilocularis: developmental stage-specific expression of Antigen B 8-kDa-subunits.. https://doi.org/10.1016/j.exppara.2005.12.007

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