PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 16542666

Validation of a solid phase extraction-high performance liquid chromatographic method for the determination of eprosartan in human plasma.

N Ferreirós·G Iriarte·R M Alonso·R M Jiménez·E Ortíz
View original source ↗Export metadata ↓

Abstract

In this work, a solid phase extraction-reversed phase high performance liquid chromatographic (SPE-RP-HPLC) method with photometric detection for monitoring the antihypertensive drug eprosartan has been validated in order to assure good quantitation of eprosartan in plasma samples obtained from patients under cardiovascular treatment. This analytical method was developed by using experimental design and quantitation was accomplished with the internal standard method. No interferences were observed from endogenous compounds of plasma and other drugs which are commonly co-administered in elderly patients. The recoveries of eprosartan from plasma samples, measured at three levels of the linear concentration range (150-4000 ng/mL) were found to be between 93.4 and 102.8%. The intraday and interday precision and accuracy (measured by relative standard deviation, RSD, and relative error, RE, respectively) were always lower than 13% (RSD) and 4% (RE). Stability studies showed that eprosartan stock solutions are stable for at least 3 months when stored at 8 degrees C and plasma samples containing the drug were stable at least during the whole analytical method.

Explore related subjects

AcrylatesChemical FractionationChromatography, High Pressure LiquidDrug StabilityHumansImidazolesReproducibility of ResultsSensitivity and SpecificityThiophenesJournal ArticleResearch Support, Non-U.S. Gov'tValidation Study

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

N Ferreirós, G Iriarte, R M Alonso, R M Jiménez, E Ortíz. 2006-03-20. Validation of a solid phase extraction-high performance liquid chromatographic method for the determination of eprosartan in human plasma.. https://doi.org/10.1016/j.chroma.2006.02.055

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Covalent immobilization of redox enzyme on electrospun nonwoven poly(acrylonitrile-co-acrylic acid) nanofiber mesh filled with carbon nanotubes: a comprehensive study.

In this work, novel conductive composite nanofiber mesh possessing reactive groups was electrospun from solutions containing poly(acrylonitrile-co-acrylic acid) (PANCAA) and multi-walled carbon nanotubes (MWCNTs) for redoxase immobilization, assuming that the incorporated MWCNTs could behave as electrons transferor during enzyme catalysis. The covalent immobilization of catalase from bovine liver on the neat PANCAA nanofiber mesh or the composite one was processed in the presence of EDC/NHS. Results indicated that both the amount and activity retention of bound catalase on the composite nanofiber mesh were higher than those on the neat PANCAA nanofiber mesh, and the activity increased up to 42%. Kinetic parameters, K(m) and V(max), for the catalases immobilized on the composite nanofiber mesh were lower and higher than those on the neat one, respectively. This enhanced activity might be ascribed to either promoted electron transfer through charge-transfer complexes and the pi system of carbon nanotubes or rendered biocompatibility by modified MWCNTs. Furthermore, the immobilized catalases revealed much more stability after MWCNTs were incorporated into the polymer nanofiber mesh. However, there was no significant difference in optimum pH value and temperature, thermal stability and operational stability between these two immobilized preparations, while the two ones appeared more advantageous than the free in these properties. The effect of MWCNTs incorporation on another redox enzyme, peroxidase, was also studied and it was found that the activity increased by 68% in comparison of composite one with neat preparation.

Acrylates↗
PubMed2007

The effect of finishing and polishing procedures on the surface roughness of composite resin materials.

AIMS: The aims of this study were to evaluate the effect of various finishing and polishing procedures on the surface roughness of six different composite resin materials (Artemis Enamel, TPH Spectrum, Filtek A-110, Filtek Supreme Enamel, Solitaire 2, and Filtek P-60) as well as to evaluate the effectiveness of the surface sealant application (BisCover) on the surface roughness after finishing and polishing procedures of tested composites. METHODS AND MATERIALS: Specimens (n=168) measuring 5 mm in diameter x 2 mm in thickness were fabricated in a plexiglass well covered with a Mylar strip using six composite resins. A control group of seven specimens of each material received no polishing after being cured under the Mylar strip. Twenty-one specimens for each composite were randomly divided among three finishing and polishing groups (n=7). Each group was polished using a different system: Carbide bur/Sof-Lex disc, Carbide bur/Enhance disc with polishing paste, and Carbide bur/Edenta composite finishing kit. The average surface roughness (Ra, microm) of the control and treated specimens were measured with the Mitutoyo Surftest-402 Surface Roughness tester. After a surface sealant (BisCover) was applied to all treated specimens, according to manufacturer's instructions, the average roughness (Ra) was measured again. Results were statistically analyzed using analysis of variance (ANOVA) and the post-hoc Scheffe's test at a p<0.05 significance level. RESULTS: Significant differences were found for the surface roughness (p<0.05) with interaction among composite resins and the finishing systems used (p<0.05). Enhance/Biscover finishing and polishing procedure surface was not significantly different from the Mylar strip surface groups (p>0.05). The Mylar strip group was not significantly different from the Sof-Lex/BisCover and Edenta/BisCover groups. The ranking of mean Ra values by materials was as follows: Filtek Supreme Enamel < Filtek A110 < TPH Spectrum < Artemis <Filtek P-60 < Solitaire 2. The ranking of mean Ra values by polishing systems was as follows: Enhance/BisCover < Mylar Strip < Sof-Lex/BisCover < Edenta/BisCover < Sof-Lex < Enhance < Edenta. CONCLUSION: Smoother surfaces were recorded for the Enhance/BisCover and the Mylar strip-formed surface groups. The composite finishing kit Edenta significantly increased the Ra for all tested composites (p<0.05). But after finishing with Edenta, the use of a surface sealant (BisCover) significantly improved the surface smoothness of all tested composites (p<0.05). Use of BisCover surface sealant on anterior and posterior resin composite restorations after finishing and polishing procedures is recommended.

Acrylates↗